In vitro screening of Trichoderma species against Sclerotium rolfsii Sacc. for antagonism

Abstract

In vitro study of antagonism was performed by dual culture method between the test pathogen, Sclerotium rolfsii Sacc. and different strains of Trichoderma species. Maximum inhibition of radial growth of S. rolfsii was observed due to T. harzianum BHU (90.0%) while no inhibition at all (0.0%) was recorded in case of T. virens 2194 and T. virens 3067. Based on the results obtained for the colony interaction between different strains of Trichoderma species and the test pathogen, five potent strains of Trichoderma species were selected for further study. These were as follows: T. harzianum BHU, T. harzianum IVRI, T. viride 1, T. pseudokoningii NBRI and T. virens BHU. The effect of different concentrations of culture filtrates of the above selected strains of Trichoderma species on per cent inhibition of radial growth of S. rolfsii was performed. Maximum inhibition of radial growth of S. rolfsii was observed in case of T. harzianum BHU (44.0%), while minimum inhibition of radial growth was observed by T. virens BHU (26.2%) at 40% concentration.